"I've always had the highest admiration for the Queen and the unifying role that she plays in our country and across the Commonwealth," he told.
"As for the rest, all other matters to do with the royal family, I've spent a long time now not commenting on royal family matters and I don't intend to depart from that today."
Meghan, who is biracial, described that when she was first pregnant with son Archie, there were “concerns and conversations about how dark his skin might be when he’s born.”
Markle, who is African American, says her husband Prince Harry revealed his family's concerns over Archie's skin tone, as well as the security he would be entitled to, ahead of his birth on May 6, 2019.
"The duchess is saddened by this latest attack on her character, particularly as someone who has been the target of bullying herself," her spokesman said.
In an interview with Oprah Winfrey, Harry invoked the memory of his late mother, Princess Diana, who had to find her way alone after she and Prince Charles divorced.
The news group also claims she wanted to use the hand-written letter "as part of a media strategy" and discussed it with royal communications officials before it was sent.