Some fear deep-seated rancor will continue fueling revolt in a country intimately familiar with violent conflict and deep social inequality, with many people with little to lose.
Around 8,000 people took to the streets of Medellin, Colombia's second largest city, by mid-morning, chanting slogans against Duque's political mentor, former president Alvaro Uribe.
Cali, Colombia's third-biggest city and the worst affected by the ongoing unrest, has had soldiers patrolling its streets since last Friday on government orders.
The rights office called for calm on all sides ahead of a planned mass protest on Wednesday, and stressed that security forces should only use firearms as a last resort.
On Tuesday, the country reached a new record of deaths (429) for the second day in a row, as a third wave of the pandemic threatens to swamp the hospital system.