The benchmark Nikkei 225 index slipped 0.42 percent or 113.38 points to 26,704.56 in early trade, while the broader Topix index was down 0.19 percent or 3.39 points at 1,776.03.
Two allergic reactions were reported on the first day of the rollout of the Pfizer COVID-19 vaccine, the head of Britain's medicine regulator said on Wednesday.
The S&P 500 opened lower by 8.91 points, or 0.24pc, at 3,683.05, while the Nasdaq Composite dropped 16.77 points, or 0.13pc, to 12,503.17 at the opening bell.
The MSCI world equity index dipped after Reuters reported Washington was preparing to impose sanctions on some Chinese officials over their alleged role in Beijing’s disqualification of elected opposition legislators in Hong Kong.
The US added 245,000 new jobs last month, much below the level expected by analysts, although the unemployment rate dipped to 6.7 from 6.9 percent, the lowest since the pandemic struck.
US giant Pfizer and its German partner BioNTech confirmed they would seek approval on Friday to roll out their coronavirus vaccine. They are eyeing December for final approval.
The three major indexes notched their biggest weekly percentage gains since April as the prospect of policy gridlock in Washington eased worries a Biden administration might tighten regulations on U.S. companies.
Wall Street continued its path higher through a trade war and impeachment, going on to new record highs following a deep slump caused by the coronavirus pandemic that continues to cripple the global economy.
Major indices climbed for a second straight session after US President Donald Trump walked back a cancelation of stimulus talks that rattled investors on Tuesday.