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Bieber, 18, appears as himself when trying to gain entry into a talent show where Bart Simpson is playing piano, but is denied.
Bieber apparently did a tweet about the news, "Just did a voice over for the SIMSPONS!!! #swaggy" and executive producer Al Jean has since confirmed the guest appearance.
Steve Carell, Zooey Deschanel, Anne Hathaway and Natalie Portman are also expected to lend their voices to "The Simpsons" upcoming 24th season.
The “Best Friend” singer will make his cameo appearance in “Simpson” which is expected to hit the screens in 2013.