It will be SilkAir's first Australian destination and is part of an overall plan by parent company SIA to expand services to northern Australia, the airline said in a statement.
Starting March 26, SilkAir will fly four times a week to Darwin with next-day return flights, subject to regulatory approvals.
It will use Airbus A319 and A320 aircraft that will offer economy and business cabins, SilkAir said.
"This is a very significant move for us and will complement our parent company’s ongoing efforts to better serve the Australian market, which has been given a boost by its recently approved alliance with Virgin Australia," said chief executive Marvin Tan.
SIA and Virgin Australia announced in June a tie-up that will see the two carriers share flights, coordinate schedules and undertake joint sales and marketing programmes.
But the tie-up excludes the lucrative Australia-US sector, where SIA's bid to ply the trans-Pacific routes from Australia to the US West Coast has been denied repeatedly by Canberra.
SIA is launching a long-haul budget carrier called Scoot in mid-2012 with Sydney as its first destination from Singapore, underscoring the importance of the Australian market to the group.