Gilani said his government had convinced other countries of the world through diplomatic channels that "these drone attacks are creating problems for us."
His remarks came just hours after a drone strike killed six fighters from the Al-Qaeda-linked Haqqani network on the Afghan border -- the first such strike in a month.
"Under a well-thought-out strategy we had separated the tribes from militants, but when drone attacks occur, militants and tribes unite again, which is difficult for us to sustain politically and it also create difficulties for the military operations," Gilani said.
He has called for an end to drone strikes a number of times in the past and criticised the campaign as an infringement on Pakistani sovereignty.
Gilani on Wednesday emphasised Pakistan's importance in the war in terror and fight against Taliban militants.
"Nobody can win Afghanistan war without Pakistan. Drone attacks are not in favour of both countries," he said.