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Twitter Counter, which tracks and analyzes Twitter usage, said Thursday that, according to its algorithms, the Biebs will overtake the Mother Monster around 4:23 pm (2123 GMT) on Sunday.
"For now, Lady Gaga is in the lead in follows alone," it said in an email to subscribers. "Bieber's account, however, is growing at a faster rate."
Lady Gaga, who has led the Twitter pack since August 2010 when she outpaced fellow pop star Britney Spears, had 33,159,886 followers -- and Bieber 33,102,814 -- shortly before 4:00 p.m. (2100 GMT) on Thursday.
Both are currently on tour in the United States.